During all seven chapters ofSeparate Ways, players run into multiple bosses, including the infamous U-3 that was previously cut fromResident Evil 4 Remake. But before players even begin Ada Wong’s side of the story, they may wonderhow many boss fightsawait them and the best strategies needed to defeat them all.

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This guide will answer that question while providing some useful tips thatResident Evil 4 Remakeplayers should take note of when facing the bosses in theSeparate WaysDLC.

List of All Boss Fights in the Separate Ways DLC

In the expansion ofRE4 Remake, players can expect to facefour bosses: The Black Robe, El Gigante, Test Subject, and Saddler. Of these, The Black Robe also appears in an alternative form known as Pesanta or U-3, providing an additional boss encounter to the roster.

Here’s the list of boss fights in order:

The Black Robe

The Black Robe isthe first boss fightinSeparate Waysthat players encounter in three separate locations: Church, Village, and Island.

El Gigante

El Gigante is agiant creature that attacks players in Chapter 3. To defeat him, players mustgrapple to the rooftops and keep shooting the parasite on his back. Once the giant is stunned, they must press RT (R2 on PlayStation) to perform a melee attack on his Las Plaga. Repeating this process two to three times will eradicate the monster for good.

Pesanta

Pesanta (U-3) is the ultimate form of The Black Robe, a test subject that looks like a hybrid human and scorpion. InChapter 5 of the DLC, players must face off against this formidable creature in the tunnels of Gregorio’s Waterway.

Test Subject

The Test Subject isan unknown, lizard-looking creaturethat players come across in Chapter 6 while traversing the Comms Facility.

Players must first run away from him and enter the sewage channel. However, as players make their way out of the Facility, they will have an opportunity todefeat the Test Subject in the laser room through a Quick Time Event (QTE).

image showing the second black robe encounter in the resident evil 4 separate ways dlc.

The QTE prompt sequence is B, B, B, and RB. (On PlayStation, Circle, Circle, Circle, R1.)

Saddler

Saddler is the final boss of theSeparate WaysDLC in Chapter 7. To beat him, players will need totarget the Las Plaga eye in his mouth.

Saddler will attack players with six moves: Lunge, strike, grab, ground attack, poisonous vomit, and tornado strike. The first five can be dodged and parried (B/LB or Circle/L1). However, to evade the last move, tornado lunge, players must press the grapple button before Saddler reaches them.

Verdugo Resident Evil 4 Remake Separate Ways

Resident Evil 4 Remake: Separate Waysis available now for PC, PS4, PS5, and Xbox Series X/S.

image showing the el gigante in the separate ways dlc in the re4 remake.

image showing the u-3 in chapter five of separate ways resident evil 4

image showing the test creature boss in chapter 6 of separate ways resident evil 4

image showing saddler in separate ways resident evil 4